用HPLC法测得某药保留时间为12.54min,半高峰宽0.6min,色谱柱的理论板数为A:116B:484C:2408D:2904E:2420

作者: rantiku 人气: - 评论: 0
问题 用HPLC法测得某药保留时间为12.54min,半高峰宽0.6min,色谱柱的理论板数为
选项 A:116 B:484 C:2408 D:2904 E:2420
答案 E
解析 色谱柱的理论板数计算式为:n=5.54(tR/W1/2)2,n=5.54(12.54/0.6)2=2420。

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